三种多糖对鸡胚肝细胞抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2018.07.022

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (7): 1517-1523.doi: 10.11843/j.issn.0366-6964.2018.07.022

三种多糖对鸡胚肝细胞抗氧化能力的影响

田卫军^1,2, 秦嘉玲², 岳婵娟¹, 侯冉冉¹, 刘宽辉¹, 胡元亮^1*

1. 南京农业大学中兽医学研究室, 南京 210095;
2. 富阳区畜牧兽医局, 杭州 311400

收稿日期:2017-12-20 出版日期:2018-07-23 发布日期:2018-07-23
通讯作者: 胡元亮,教授,博士生导师,主要从事中兽医学研究,E-mail:ylhu@njau.edu.cn
作者简介:田卫军(1991-),男,江苏南京人,硕士,主要从事中兽医学研究,E-mail:2014107115@njau.edu.cn
基金资助:
国家自然科学基金（31272596）；公益性行业（农业）科研专项（201403051）

Effects of Three Kinds of Polysaccharides on Antioxidative Ability of Chicken Embryo Liver Cells

TIAN Wei-jun^1,2, QIN Jia-ling², YUE Chan-juan¹, HOU Ran-ran¹, LIU Kuan-hui¹, HU Yuan-liang^1*

1. Institute of Traditional Chinese Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. Animal Husbandry and Veterinary Authority of Fuyang District, Hangzhou 311400, China

Received:2017-12-20 Online:2018-07-23 Published:2018-07-23

摘要/Abstract

摘要：

为了研制抗氧化中药多糖制剂，比较了枸杞多糖（LBP）、白术多糖（AMP）和当归多糖（CAP）对鸡胚肝细胞（CEL）的抗氧化能力的影响，以维生素C（VC）为对照。首先测定了4个药对CEL的安全浓度，然后取安全浓度范围内3种浓度的各药分别加入到CEL的培养体系中，培养24 h后加入H₂O₂攻击1 h，测定细胞活力（A_{570 nm}）、细胞内ROS含量、GSH-Px和SOD的活性。结果显示，H₂O₂组的A_{570 nm}值最小、ROS含量最高、GSH-Px和SOD活性最低，均与细胞对照组的差异显著（P<0.05）；3个多糖组和VC组的A_{570 nm}值均大于或显著大于、ROS含量均低于或显著低于、GSH-Px和SOD活性均高于或显著高于H₂O₂组，高浓度的作用最显著（P<0.05）；LBP组3个浓度的作用均强于同浓度的AMP组和CAP组，相当于甚至优于VC。结果表明，3种多糖均具有较强的抗氧化活性，能够抵抗H₂O₂攻击引起的鸡胚肝细胞活力降低、细胞内ROS含量升高和抗氧化酶活性降低，从而增强鸡胚肝细胞的抗氧化能力，LBP的作用最强，可以考虑作为多糖类抗氧化剂。

Abstract:

In order to develop the antioxidative polysaccharide preparations, the effects of Lycum barbarum polysaccharide(LBP), Atractylodes macrocephala polysaccharide(AMP) and Chinese Angelica polysaccharide(CAP), on resisting oxidative damage ability of Chicken embryo liver cells(CEL) were compared, vitamin C(VC) were used as control. The safe concentrations of four drugs on CEL were determined. Then they at three concentrations within safe concentrations were respectively added into cultivation system of CEL. After cultivation of 24 hours, the cells were attacked by H₂O₂ for an hour, the cellular vitality(A_{570 nm} value), intra-cellular ROS content, GSH-Px and SOD activities were assayed. The result showed that the A_{570 nm} values in H₂O₂ group were the lowest, ROS contents were the highest and the activities of GSH-Px and SOD were the lowest, which there were significant differences as compared with cell control group(P<0.05). The A_{570 nm} values in 3 polysaccharides and VC groups were higher or significantly higher, ROS contents were lower or significantly lower and the activities of GSH-Px and SOD were higher or significantly higher than those in H₂O₂ group, especially in high concentrations(P<0.05), the actions were most significant. The actions of LBP at 3 concentrations were stronger than those of AMP and CAP at same concentration, and equivalent even superior to VC. The results indicate that three kinds of polysaccharides all possess stronger antioxidant activity, can resist the CEL vitality depress, intra-cellular ROS contents elevation and antioxidase activities decline caused by H₂O₂ attack, thus strengthen CEL ability of oxidation resistance, LBP has the strongest activity and can be considered as polysaccharid antioxidant.

中图分类号:

S853.7

田卫军, 秦嘉玲, 岳婵娟, 侯冉冉, 刘宽辉, 胡元亮. 三种多糖对鸡胚肝细胞抗氧化能力的影响[J]. 畜牧兽医学报, 2018, 49(7): 1517-1523.

TIAN Wei-jun, QIN Jia-ling, YUE Chan-juan, HOU Ran-ran, LIU Kuan-hui, HU Yuan-liang. Effects of Three Kinds of Polysaccharides on Antioxidative Ability of Chicken Embryo Liver Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1517-1523.

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三种多糖对鸡胚肝细胞抗氧化能力的影响

Effects of Three Kinds of Polysaccharides on Antioxidative Ability of Chicken Embryo Liver Cells

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